Results
| PMID | 12840897 |
| Gene Name | CCL2 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 15 |
| Population details | 15 patients with endometriosis |
| Sex | Female |
| Associated genes | IL-1 beta, TNF-alpha, MCP-1 |
| Other associated phenotypes |
Endometriosis |
|
J Tongji Med Univ. 1999;19(3):212-4. Gao, Y| Luo, L| He, F Department of Obstetrics and Gynecology, Xiehe Hospital, Tongji Medical University, Wuhan 430022. To investigate the clinical significance of monocyte chemotactic protein-1 (MCP-1) produced by endometriotic tissues, the endometriotic tissues were taken from 15 patients with endometriosis. MCP-1 mRNA and MCP-1 protein were determined by dot blot analysis and enzyme linked immunosorbent assay (ELISA) in endometriotic cells cultured with or without interleukin-1 beta (IL-1 beta, 2 micrograms/L), tumor necrosis factor-alpha (TNF-alpha, 20 g/L). After exposure to IL-1 beta or TNF-alpha, the expression of MCP-1 mRNA in the endometriotic cells (8.635 +/- 0.826, 7.031 +/- 0.970, respectively) were significantly higher than that in the control group (4.482 +/- 0.435, P < 0.05); The expression of MCP-1 protein in IL-1 beta and TNF-alpha group was 4.52 +/- 0.09 micrograms/L, 2.87 +/- 0.27 micrograms/L, respectively, which were significantly higher than 1.74 +/- 0.16 micrograms/L in control (P < 0.01). The results suggested that IL-1 beta and TNF-alpha could up-regulate the expression of MCP-1 in endometriotic cells, which might be related to the development of endometriosis. Mesh Terms: Cells, Cultured| Chemokine CCL2/*biosynthesis/genetics| Culture Media, Conditioned| Endometriosis/*metabolism/pathology| Endometrium/*metabolism/pathology| Female| Humans| Interleukin-1/*pharmacology| Peritoneal Diseases/metabolism/pathology| Tum |
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